Kev lag luam sterility ntawm cov khoom noj kaus poom yog hais txog lub xeev tsis muaj menyuam uas tsis muaj cov kab mob pathogenic thiab cov kab mob uas tsis muaj kab mob uas tuaj yeem rov ua dua tshiab hauv cov kaus poom tom qab cov kaus poom tau txais kev kho cua sov kom tsis muaj menyuam, yog qhov tseem ceeb ua ntej rau cov khoom noj kaus poom kom ua tiav. lub neej txee ntev dua los ntawm kev ua kom cov zaub mov muaj kev nyab xeeb thiab zoo. Kev lag luam sterility ntawm cov kaus poom hauv cov khoom noj microbiological kuaj yog tus cwj pwm los ntawm cov txheeb ze tsis muaj menyuam, tsis muaj kab mob pathogenic, thiab tsis muaj kab mob uas tuaj yeem sib npaug hauv cov kaus poom ntawm chav tsev kub.
Txhawm rau kom ua tiav cov qauv kev lag luam ua kom tsis muaj menyuam, cov txheej txheem tsim khoom noj hauv cov kaus poom feem ntau suav nrog cov txheej txheem xws li raw khoom pretreatment, canning, sealing, kom tsis muaj menyuam, thiab ntim. Cov neeg tsim khoom nrog ntau cov cuab yeej tsim khoom thiab kev tswj xyuas kom zoo dua muaj cov txheej txheem ntau lawm thiab zoo meej.
Kev lag luam kaus poom sterility tshuaj xyuas tshuab hauv kev tshuaj xyuas cov khoom noj microbiological tau ua tiav, thiab kev tshuaj xyuas ntawm nws cov txheej txheem tshwj xeeb yog qhov tsim nyog rau kev siv cov thev naus laus zis no hauv kev ua haujlwm kom ntseeg tau cov zaub mov kev nyab xeeb ntawm cov kaus poom zaub mov. Cov txheej txheem tshwj xeeb ntawm cov kaus poom kev lag luam sterility tshuaj xyuas hauv cov khoom noj microbiological tshuaj xyuas yog raws li hauv qab no (qee qhov kev soj ntsuam nruj dua lwm tus neeg tuaj yeem muaj ntau yam tshuaj xyuas):
1. Cov kab mob hauv kaus poom
Cov kab lis kev cai hauv kaus poom yog ib qho ntawm cov txheej txheem tseem ceeb hauv kev lag luam sterility tshuaj xyuas cov khoom noj kaus poom. Los ntawm kev cob qhia cov ntsiab lus ntawm cov kaus poom cov qauv, thiab tshuaj xyuas thiab tshuaj xyuas cov kab mob kab mob hauv cov kab mob, cov khoom siv microbial hauv cov kaus poom tuaj yeem ntsuas.
Cov kab mob pathogenic hauv cov kaus poom muaj xws li tab sis tsis txwv rau cov kab mob thermophilic, xws li Bacillus stearothermophilus, Bacillus coagulans, Clostridium saccharolyticus, Clostridium niger, thiab lwm yam; cov kab mob mesophilic anaerobic, xws li botulinum toxin Clostridium, Clostridium spoilage, Clostridium butyricum, Clostridium pasteurianum, thiab lwm yam. Mesophilic aerobic kab mob, xws li Bacillus subtilis, Bacillus cereus, thiab lwm yam.; Cov kab mob uas tsis tsim cov kab mob xws li Escherichia coli, Streptococcus, poov xab Thiab pwm, kub-resistant pwm thiab hais txog. Ua ntej nqa cov kab mob hauv cov kaus poom, nco ntsoov ntsuas pH ntawm lub kaus poom txhawm rau xaiv qhov nruab nrab tsim nyog.
2. Kev kuaj cov khoom siv
Txoj kev sampling feem ntau yog siv los ua piv txwv ntawm kev sim cov khoom ntawm cov kaus poom zaub mov. Thaum kuaj cov khoom loj ntawm cov kaus poom, cov qauv feem ntau yog ua raws li cov khoom lag luam xws li cov chaw tsim khoom, cov cim lag luam, ntau yam, qhov chaw ntawm cov kaus poom zaub mov lossis lub sijhawm tsim khoom. Rau cov kaus poom txawv txav xws li cov kaus poom rusted, cov kaus poom deflated, dents, thiab o nyob rau hauv cov kev lag luam ntawm cov tub lag luam thiab cov warehouses, cov qauv tshwj xeeb yog feem ntau ua raws li qhov xwm txheej. Nws yog ib qho tseem ceeb uas yuav tsum tau ua piv txwv ntawm cov khoom siv sim los xaiv cov qauv tsim nyog raws li qhov xwm txheej tiag tiag, kom tau txais cov ntaub ntawv sim uas cuam tshuam txog qhov zoo ntawm cov kaus poom zaub mov.
3. Khaws tus qauv
Ua ntej khaws cov qauv, kev ua haujlwm xws li qhov hnyav, ua kom sov, thiab qhib cov kaus poom yuav tsum tau ua. Ntsuas qhov hnyav ntawm lub peev xwm cais, nyob ntawm seb hom tuaj yeem, nws yuav tsum yog 1g lossis 2g. Ua ke nrog pH thiab kub, cov kaus poom tau khaws cia ntawm qhov kub thiab txias rau 10 hnub; cov kaus poom uas muaj roj los yog leaked thaum lub sij hawm txheej txheem yuav tsum tau muab tshem tawm tam sim ntawd rau kev soj ntsuam. Tom qab cov txheej txheem khaws cia kub dhau lawm, tso lub peev xwm ntawm chav tsev kub rau aseptic qhib. Tom qab qhib lub kaus poom, siv cov cuab yeej tsim nyog coj 10-20 mg ntawm cov ntsiab lus ua ntej hauv lub xeev tsis muaj menyuam, hloov mus rau hauv lub thawv tsis muaj menyuam, thiab khaws cia hauv lub tub yees.
4.Tsawg acid noj kab lis kev cai
Kev cog qoob loo ntawm cov khoom noj uas tsis muaj kua qaub yuav tsum muaj txoj hauv kev tshwj xeeb: cog qoob loo ntawm brompotassium liab doog ntawm 36 ° C, cog qoob loo ntawm brompotassium liab doog ntawm 55 ° C, thiab cog cov nqaij nruab nrab ntawm 36 ° C. Cov txiaj ntsig tau smeared thiab stained, thiab kev tshuaj ntsuam xyuas meej dua yog teem rau tom qab kuaj xyuas kab mob, thiaj li ua kom lub hom phiaj raug ntawm cov kab mob kev txheeb xyuas cov kab mob hauv cov zaub mov uas muaj kua qaub. Thaum culturing nyob rau hauv nruab nrab, tsom ntsoov rau kev soj ntsuam cov kua qaub ntau lawm thiab roj zus tau tej cov microbial colonies nyob rau nruab nrab, nrog rau cov tsos thiab xim ntawm lub colony, thiaj li yuav paub meej tias cov microbial hom nyob rau hauv cov zaub mov.
5. Kev tshuaj xyuas microscopic
Kev soj ntsuam microscopic smear yog feem ntau siv thawj txoj kev tshuaj ntsuam xyuas rau cov kaus poom kev lag luam tsis muaj menyuam, uas yuav tsum tau muaj kev paub txog kev soj ntsuam kom tiav. Nyob rau hauv ib puag ncig tsis muaj menyuam, siv aseptic ua haujlwm, smear cov kab mob ua kua ntawm cov kab mob uas muaj nyob rau hauv cov kaus poom cov qauv uas tau raug coj los ntawm qhov ntsuas kub tsis tu ncua hauv nruab nrab, thiab saib xyuas cov tsos mob ntawm cov kab mob hauv qab lub tshuab hluav taws xob siab, thiaj li. txiav txim siab hom kab mob hauv cov kab mob ua kua. Kev tshuaj ntsuam xyuas, thiab npaj cov kauj ruam tom ntej ntawm cov kab lis kev cai thiab kev txheeb xyuas kom paub meej ntxiv txog hom kab mob uas muaj nyob hauv lub kaus poom. Cov kauj ruam no yuav tsum muaj kev ua haujlwm siab heev ntawm cov neeg soj ntsuam, thiab kuj tau dhau los ua qhov txuas uas tuaj yeem kuaj tau qhov kev paub zoo tshaj plaws thiab kev txawj ntse ntawm cov neeg soj ntsuam.
6. Kev sim cog qoob loo rau cov zaub mov acidic nrog pH qis dua 4.6
Rau cov khoom noj acidic nrog tus nqi pH qis dua 4.6, feem ntau tsis tas yuav tsum tau kuaj cov kab mob tshuaj lom. Nyob rau hauv cov txheej txheem kev cog qoob loo tshwj xeeb, ntxiv rau kev siv cov khoom siv acidic broth raws li qhov nruab nrab, nws tseem yuav tsum tau siv malt extract broth ua qhov nruab nrab rau kev cog qoob loo. Los ntawm smearing thiab microscopic kuaj ntawm kab lis kev cai kab lis kev cai, cov hom kab mob hauv cov kua qaub tuaj yeem txiav txim siab, txhawm rau ua kom lub hom phiaj ntxiv thiab qhov tseeb kev ntsuas ntawm cov zaub mov kev nyab xeeb ntawm cov kua qaub.
Post lub sij hawm: Aug-10-2022